ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibiting action of transforming growth factor-beta type R II receptor(TGF-beta R II) antibody to fibroblasts.</p><p><b>METHODS</b>Four groups of cultured skin fibroblasts were treated with different dosages of TGF-beta R II antibody. Examinations were performed with HYP, MTT, microscope, and flow cytometry techniques.</p><p><b>RESULTS</b>TGF-beta R II antibody resulted in significant inhibition of fibroblasts(P < 0.01).</p><p><b>CONCLUSION</b>TGF-beta R II antibody has inhibiting effects on the proliferation of skin fibroblasts and with a dose-dependent manner.</p>
Subject(s)
Adolescent , Child , Humans , Antibodies, Monoclonal , Pharmacology , Cell Division , Cells, Cultured , Fibroblasts , Cell Biology , Flow Cytometry , Microscopy, Electron , Protein Serine-Threonine Kinases , Receptors, Transforming Growth Factor beta , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>To explore a method for reconstruction of Poland's chest wall deformity.</p><p><b>METHODS</b>The customized, textured silicone prosthesis was fabricated from a soft silicone polymer that approximated to the softness of the pectoralis major muscle. A lateral incision of 6 cm on the affected chest was made. After a subcutaneous pocket above the ribs was created through the incision, the prosthesis was placed in the pocket.</p><p><b>RESULTS</b>Since October 2000, chest wall reconstruction for Poland's syndrome deformity has been performed in 7 male patients (18 to 45 years) with the customized soft silicone prosthesis. Follow-up for 3 to 17 months showed that all patients were satisfied with the aesthetical results. Seroma occurred in 2 patients, and needle aspiration was used to solve the problem.</p><p><b>CONCLUSION</b>This method is simple and less traumatic. The reconstructed chest wall approximates to the softness and appearance of the contralateral pectoralis muscle.</p>